Micellar Hyaluronidase and Spiperone as a Potential Treatment for Pulmonary Fibrosis

Concentration of hyaluronic acid (HA) in the lungs increases idiopathic pulmonary fibrosis (IPF). HA is involved organization fibrin, fibronectin, and collagen. has been proposed to be a biomarker potential target for antifibrotic therapy. Hyaluronidase (HD) breaks down into fragments, but subject rapid hydrolysis. A conjugate poloxamer hyaluronidase (pHD) was prepared using protein immobilization with ionizing radiation. In model bleomycin-induced fibrosis, pHD decreased level tissue IL-1β TGF-β, prevented infiltration lung parenchyma by CD16+ cells, reduced perivascular peribronchial inflammation. Simultaneously, decrease concentrations HA, hydroxyproline, collagen 1, total soluble collagen, area connective observed. The effects were significantly stronger compared native HD which can attributed higher stability pHD. Additional spiperone administration increased anti-inflammatory accelerated regeneration damaged lung. potentiating explained disruption dopamine-induced mobilization migration fibroblast progenitor cells differentiation mesenchymal stem (MSC) stromal lines. Thus, combination may represent promising approach treatment IPF regeneration.